منابع مشابه
Molecular interactions in the RNA bacteriophage MS2.
The simple RNA bacteriophages of E.coli have long been used as ideal model systems in which the details of RNA replication, translation and the control of gene expression can be studied in molecular detail’.’. In particular, the translational repression of the phage replicase cistron by coat protein subunits has attracted a great deal of attention and this has become a paradigm for sequence-spe...
متن کاملThe allosteric switching mechanism in bacteriophage MS2.
We use all-atom simulations to elucidate the mechanisms underlying conformational switching and allostery within the coat protein of the bacteriophage MS2. Assembly of most icosahedral virus capsids requires that the capsid protein adopts different conformations at precise locations within the capsid. It has been shown that a 19 nucleotide stem loop (TR) from the MS2 genome acts as an allosteri...
متن کاملDirect Evidence for Packaging Signal-Mediated Assembly of Bacteriophage MS2
Using cross-linking coupled to matrix-assisted laser desorption/ionization mass spectrometry and CLIP-Seq sequencing, we determined the peptide and oligonucleotide sequences at the interfaces between the capsid proteins and the genomic RNA of bacteriophage MS2. The results suggest that the same coat protein (CP)-RNA and maturation protein (MP)-RNA interfaces are used in every viral particle. Th...
متن کاملEncapsidation of heterologous RNAs by bacteriophage MS2 coat protein.
The RNA bacteriophages of E. coli specifically encapsidate a single copy of the viral genome in a protein shell composed mainly of 180 molecules of coat protein. Coat protein is also a translational repressor and shuts off viral replicase synthesis by interaction with a RNA stem-loop containing the replicase initiation codon. We wondered whether the translational operator also serves as the vir...
متن کاملSurvival of Bacteriophage MS2 on Filtering Facepiece Respirator Coupons
The reuse of filtering facepiece respirators (FFRs) after decontamination has been suggested as a strategy to conserve supplies during an influenza pandemic. The feasibility of decontaminating FFRs has been investigated under laboratory conditions; however, the need for decontamination of FFRs is not well characterized. In this study the potential for FFRs to act as fomites was examined using b...
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ژورنال
عنوان ژورنال: Structure
سال: 2003
ISSN: 0969-2126
DOI: 10.1016/j.str.2003.09.021